Influenza complement-fixation. A simple quantitative micro-method

Quantitative micro-complement fixation test.

A quantitative micro-complement fixation test capable of detecting nanogram quantities of antigen is described. The test is simple to perform and is highly reproducible. Typical results from three antigen-antibody systems are given.

Micro-complement fixation: a quantitative estimator of protein evolution.

The quantitative immunological technique of micro-complement fixation (MC'F) has been routinely used during the past decade to assess evolutionary relationships among living vertebrate species. The large data base that has been generated, along with the excellent correlations between immunologically measured genetic distances and paleontologically derived estimates of divergence times, have for...

Micro-complement fixation in Klebsiella classification.

The alkaline phosphatases of 29 strains of bacteria assigned by various authors to the genera Aerobacter, Klebsiella and Enterobacter were compared by the micro-complement fixation technique. On the basis of phosphatase resemblance, we recommend that all strains hitherto assigned to Aerobacter aerogenes and Enterobacter aerogenes be assigned to the genus Klebsiella.

A new rapid method for automatic complement-fixation tests.

Several automatic methods have been described for complement-fixation tests. However, each of these methods presents some inconvenience either due to cross contamination or to limited sensitivity. The new method consists basically in separating clearly the fixation stage from the haemolytic stage by using two different sampler plates placed on the same sampler. By this procedure, it becomes pos...

Indirect Complement Fixation in Foot-and-Mouth Disease